5400 Manual LiCor Calibration/ Audit

 

Purpose:  Follow the steps below to calibrate and audit the CO2 LiCor sensor on the 5400 instrument.

 

Required Equipment:  Not applicable.

 

Reference:  SOPMAN

 

 

 

  1. You must first suspend serial polling from SiteManager.  To do so go to the Setup Menu and select Serial Control.
  2. Click on the 5400 line in the Device window – then change the Status setting in the Config Box to Off.  Click the Save Config button to save the change.
  3. Open the output valve on each of your 5400 calibration cylinders.  Confirm the output pressure is between 2 and 3 psi while gas is flowing.
  4. From the main screen press menu
  5. Select service mode and press enter.
  6. Select calibration audit.
  7. Press F2 for CO2 calibration.
  8. Press F2 again for auto calibration.
  9. Bottles connected will be asked by the instrument, select yes.
  10. This enters the data into the audit data file for your download interval.
  11. Perform the LiCor calibration steps listed below.
  12. From the 5400 service menu select "Manual Motion Tests" and press "MFCPres".  Move the cursor to "Circ Pump" with the arrow keys and press "On/Off".  CircPump: OFF should change to CircPump: ON.
  13. From the 5400 service menu select "Manual Motion Tests" and press "Valves".
  14. Move the cursor to "Purge" and press the "On/Off" key.  Purge: Off should change to Purge: On.
  15. Move the cursor to the N2: OFF selection with the arrow keys and press "On/Off" – N2: OFF should change to N2: ON.  CO2 Free Air is now flowing – allow the system to purge for 2-3 minutes or until the CO2 reading is stable.
  16. After the CO2 reading on the Li800 screen has stabilized record the value as the "As Found" value for the zero on the form.  Press the "On/Off" key on the Manual Motion Test screen to turn return N2: ON to N2: OFF.
  17. Move the cursor to the CO2Low: OFF selection with the arrow keys and press "On/Off" – CO2Low: OFF should change to CO2Low: ON.  CO2 low gas is now flowing – allow the system to purge for 2-3 minutes or until the CO2 reading is stable.
  18. After the CO2 reading on the Li800 screen has stabilized record the value as the "As Found" value for the CO2 Low on the form.  Press the "On/Off" key on the manual motion test screen to return CO2Low: ON to CO2Low: Off.
  19. Move the cursor to the CO2High: OFF selection with the arrow keys and press "On/Off" – CO2 High: OFF should change to CO2 High: ON.  CO2 High gas is now flowing – allow the system to purge for 2-3 minutes or until the CO2 reading is stable.
  20. After the CO2 reading on the Li800 screen has stabilized record the value as the "As Found" value for the CO2 High on the form.  Press the "On/Off" key on the manual motion test screen to turn return CO2 High: ON to CO2High: OFF.
  21. Repeat steps 1.9, 1.10, and 1.11.  When the CO2 value has stabilized in the Li800 Program select "View" and the "Calibration" from the menu.  When the calibration window opens click the "Zero" button and wait for the LI800 program to reset the zero.  When it has finished record the new zero value as the "As Left" value for the CO2 Free Air on the form.  Change NO2 back to OFF.
  22. Repeat steps 1.13.  When the CO2 value has stabilized in the Li800 Program record the value as the "As Left" value for the CO2 Low on the form.
  23. Repeat steps 1.15. When the CO2 value has stabilized in the Li800 Program select "View" and the "Calibration" from the menu.  When the calibration window enter the CO2 high cylinder value (in ppm) into the blank provided in the Li800 calibration window, click the "Span" button and wait for the LI800 program to reset the span.  When it has finished record the new span value as the "As Left" value for the CO2 High on the form.
  24. Remove the LiCor calibration RS-232 cable from the 5400 and reattach the rainbow colored RS-232 connector to the LiCor port.  Push the 5400 bulkhead back into the instrument, reattach the sampling line and close the 5400 door.
  25. From the 5400 service menu select "Manual Motion Tests" and press "MFCPres".  Move the cursor to "Circ Pump" with the arrow keys and press "On/Off".  CircPump: ON should change to CircPump: OFF.
  26. From the 5400 service menu select "Manual Motion Tests" and press "Valves".
  27. Move the cursor to "Purge" and press the "On/Off" key.  Purge: ON should change to Purge: OFF
  28. Repeat steps 1 – 6 to record a post-calibration audit into the Audit data file on the 5400.
  29. Shut off all three 5400 calibration cylinders by turning off the output valve on the regulators.
  30. You must now restart serial polling from SiteManager.  To do so go to the Setup Menu and select Serial Control.
  31. Click on the 5400 line in the Device window – then change the Status setting in the Config Box to ON.  Click the Save Config button to save the change.